Manual Automated DNA Sequencing and Analysis

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The fluorescent dye is not an environmentally hazardous chemical and has no special handling or disposal requirements. Instead of using X-ray film to read the sequence, a laser is used to stimulate the fluorescent dye. The fluorescent emissions are collected on a charge coupled device that is able to determine he wavelength.

Varying degrees of automation are also available.

Automated DNA Sequencing and Analysis

For full automation, all that is required is to load a sample tray with template DNA; the equipment performs the labeling and analysis. Abstract The Human Genome Initiative is a complex, multifaceted, international effort to establish a massive data base of map and sequence information for humans and other organisms. Cited by View all 10 citing articles. Online access to the content you have requested requires one of the following:.


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Genome, , Vol. If you have an individual subscription to this journal, or if you have purchased this article through Pay-Per-view , you can gain access by logging in with your username and password here: Username:. The Assemble by Name tool allows you to choose a portion of the fragment name to act as a shared identifier, or "assembly handle.

Automated DNA sequencing and analysis of the human genome.

Sequencher even supports Regular Expression matching for setting up the unique IDs! For example, with the click of a button you can convert 90 files, 45 pairs of forward and reverse sequences, into 45 contigs named according to your Patient IDs.

Applied Biosystems integrated systems for genetic analysis

A change in your sequence assembly parameters regroups your fragments, so you can assemble the contigs according to Clone ID, Date, Primer, or any other characteristic you record in your sequence names. Assemble by Name is particularly helpful if you do a lot of sequencing and if you have numerous samples that are done with a standard set of sequencing primers.

Some other applications of Assemble by Name can include:.